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Adi Haber, Sivan Friedman, Lior Lobel, Tamar Burg-Golani, Nadejda Sigal, Jessica Rose, Nurit Livnat-Levanon, Oded Lewinson, Anat A. Herskovits
L-glutamine Induces Expression of Listeria monocytogenes Virulence Genes
www.plospathogens.org
www.plospathogens.org
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0.83 0.64 0.02 0 k
(21)Tj
0 g
(].)Tj
2.0466 0 Td
(Using)Tj
2.5682 0 Td
(a)Tj
0.652 0 Td
(reporter)Tj
3.4526 0 Td
(cell)Tj
1.5363 0 Td
(line)Tj
1.6838 0 Td
(featuring)Tj
3.7871 0 Td
(type)Tj
1.8935 0 Td
(I)Tj
-35.2287 -1.2983 Td
(interferon-dependent)Tj
8.7363 0 Td
(luciferase)Tj
3.9231 0 Td
(expression)Tj
4.405 0 Td
([)Tj
0.83 0.64 0.02 0 k
(22)Tj
0 g
(],)Tj
2.0523 0 Td
(we)Tj
1.3152 0 Td
(screened)Tj
3.6737 0 Td
(approximately)Tj
5.896 0 Td
(5,000)Tj
/F14 1 Tf
2.3188 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F14 1 Tf
0.9637 0 Td
[()6( )11(\n)6( )4()-9()-15()11( )]TJ
-33.2841 -1.2983 Td
[(\r)2()2(\n)6()8()]TJ
/F8 1 Tf
2.245 0 Td
(mutants)Tj
3.4639 0 Td
(for)Tj
1.3663 0 Td
(decreased)Tj
4.0818 0 Td
(induction)Tj
4.0819 0 Td
(of)Tj
1.0035 0 Td
(IFN-)Tj
/F15 1 Tf
()Tj
/F8 1 Tf
2.6305 0 Td
(response.)Tj
3.9061 0 Td
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3.2145 0 Td
(the)Tj
1.4513 0 Td
(identified)Tj
4.0025 0 Td
(mutants)Tj
3.4639 0 Td
(were)Tj
-34.9112 -1.3039 Td
(several)Tj
2.8913 0 Td
(well-established)Tj
6.4516 0 Td
(virulence)Tj
3.8551 0 Td
(determinants)Tj
5.4651 0 Td
(of)Tj
/F14 1 Tf
1.0035 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F14 1 Tf
0.9638 0 Td
[()6( )11(\n)6( )4()-9()-15()11( )2(\r)2()2(\n)6()8()]TJ
/F8 1 Tf
6.0037 0 Td
(\()Tj
/F14 1 Tf
[()]TJ
/F8 1 Tf
(.)Tj
/F14 1 Tf
[(\r)]TJ
/F8 1 Tf
(.,)Tj
2.0012 0 Td
(PrfA\),)Tj
2.6533 0 Td
(as)Tj
1.0034 0 Td
(well)Tj
1.7972 0 Td
(as)Tj
1.0034 0 Td
(newly)Tj
-35.0926 -1.2982 Td
(identified)Tj
3.9968 0 Td
(potential)Tj
3.7077 0 Td
(virulence)Tj
3.8494 0 Td
(factors)Tj
2.863 0 Td
([)Tj
0.83 0.64 0.02 0 k
(21)Tj
0 g
(].)Tj
2.0466 0 Td
(One)Tj
1.9218 0 Td
(of)Tj
1.0035 0 Td
(these)Tj
2.2223 0 Td
(genes,)Tj
/F14 1 Tf
2.6306 0 Td
[()8()]TJ
/F8 1 Tf
(,)Tj
5.8677 0 Td
(which)Tj
2.6191 0 Td
(encodes)Tj
3.3959 0 Td
(a)Tj
-36.1244 -1.2983 Td
(polypeptide)Tj
4.8528 0 Td
(that)Tj
1.7518 0 Td
(is)Tj
0.8391 0 Td
(a)Tj
0.6463 0 Td
(fusion)Tj
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(of)Tj
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(a)Tj
0.6519 0 Td
(substrate-binding)Tj
7.183 0 Td
(protein)Tj
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(\(SBP\))Tj
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(and)Tj
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(a)Tj
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(domain)Tj
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(\(TMD\))Tj
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(of)Tj
1.0034 0 Td
(an)Tj
1.1906 0 Td
(ATP)Tj
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(Binding)Tj
3.3846 0 Td
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3.4355 0 Td
(\(ABC\))Tj
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(transporter,)Tj
4.8755 0 Td
(was)Tj
1.6838 0 Td
(chosen)Tj
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(further)Tj
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(analysis.)Tj
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(ABC)Tj
-34.3556 -1.2983 Td
(transporters)Tj
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(comprise)Tj
3.8607 0 Td
(a)Tj
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(large)Tj
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(super-family)Tj
5.21 0 Td
(of)Tj
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(proteins)Tj
3.4639 0 Td
(that)Tj
1.7574 0 Td
(couple)Tj
2.829 0 Td
(the)Tj
1.4513 0 Td
(energy)Tj
2.8687 0 Td
(of)Tj
1.0034 0 Td
(ATP)Tj
2.058 0 Td
(hydrolysis)Tj
-33.2955 -1.2983 Td
(to)Tj
1.0148 0 Td
(the)Tj
1.4513 0 Td
(translocation)Tj
5.3858 0 Td
(of)Tj
1.0034 0 Td
(molecules)Tj
4.1442 0 Td
(across)Tj
2.6419 0 Td
(biological)Tj
4.0309 0 Td
(membranes)Tj
4.8698 0 Td
(against)Tj
2.9877 0 Td
(their)Tj
2.0807 0 Td
(concentration)Tj
5.7372 0 Td
(gra-)Tj
-35.3477 -1.3039 Td
(dient)Tj
2.2563 0 Td
([)Tj
0.83 0.64 0.02 0 k
(23)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(25)Tj
0 g
(].)Tj
3.4979 0 Td
(ABC)Tj
2.126 0 Td
(transporters)Tj
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(have)Tj
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(been)Tj
2.0976 0 Td
(shown)Tj
2.8176 0 Td
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1.0148 0 Td
(be)Tj
1.1395 0 Td
(involved)Tj
3.6 0 Td
(in)Tj
1.0262 0 Td
(bacterial)Tj
3.5716 0 Td
(virulence)Tj
3.8494 0 Td
(and)Tj
-34.0438 -1.2982 Td
(pathogenesis,)Tj
5.4991 0 Td
(as)Tj
1.0035 0 Td
(they)Tj
1.8992 0 Td
(allow)Tj
2.3074 0 Td
(the)Tj
1.4513 0 Td
(bacteria)Tj
3.3335 0 Td
(to)Tj
1.0148 0 Td
(acquire)Tj
3.1294 0 Td
(essential)Tj
3.532 0 Td
(nutrients)Tj
3.8097 0 Td
(from)Tj
2.1827 0 Td
(the)Tj
1.4513 0 Td
(host)Tj
1.8879 0 Td
([)Tj
0.83 0.64 0.02 0 k
(26)Tj
0 g
()Tj
0.83 0.64 0.02 0 k
(32)Tj
0 g
(].)Tj
-0.004 Tc
-31.3056 -1.2983 Td
(In)Tj
1.1111 0 Td
(this)Tj
1.6725 0 Td
(work,)Tj
2.5058 0 Td
(we)Tj
1.3266 0 Td
(analyze)Tj
3.1748 0 Td
(the)Tj
1.4683 0 Td
(function)Tj
3.6283 0 Td
(of)Tj
1.0148 0 Td
(the)Tj
/F14 1 Tf
1.474 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F14 1 Tf
0.9695 0 Td
[()6( )11(\n)6( )4()-9()-15()11( )2(\r)2()2(\n)6()8()]TJ
/F8 1 Tf
6.0831 0 Td
(ABC)Tj
2.143 0 Td
(transporter)Tj
4.7224 0 Td
(GlnPQ,)Tj
3.2655 0 Td
(and)Tj
-35.7559 -1.3039 Td
(show)Tj
2.296 0 Td
(its)Tj
1.1452 0 Td
(importance)Tj
4.8245 0 Td
(in)Tj
1.0318 0 Td
(interpreting)Tj
5.0173 0 Td
(a)Tj
0.652 0 Td
(newly)Tj
2.5738 0 Td
(identified)Tj
4.0592 0 Td
(signal)Tj
2.5398 0 Td
(that)Tj
1.7745 0 Td
(induces)Tj
3.2938 0 Td
(the)Tj
1.4684 0 Td
(expression)Tj
4.4617 0 Td
(of)Tj
1.0148 0 Td
(vir-)Tj
-36.1528 -1.2983 Td
(ulence)Tj
2.8119 0 Td
(genes:)Tj
2.6645 0 Td
(L-glutamine.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 480.1889 cm
BT
/F3 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(Results)Tj
/F0 1 Tf
0 -1.4976 Td
(Deletion)Tj
3.8362 0 Td
(of)Tj
/F7 1 Tf
1.0441 0 Td
(LMRG_022701)Tj
/F0 1 Tf
7.4741 0 Td
(genes)Tj
2.9102 0 Td
(leads)Tj
2.5796 0 Td
(to)Tj
1.0441 0 Td
(a)Tj
0.7701 0 Td
(reduced)Tj
3.7843 0 Td
(type)Tj
2.0929 0 Td
(I)Tj
0.4961 0 Td
(interferon)Tj
-26.0317 -1.1669 Td
(response)Tj
4.2803 0 Td
(in)Tj
0.9874 0 Td
(macrophage)Tj
5.7119 0 Td
(cells)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 431.2062 cm
BT
/F14 1 Tf
1 TL
-0.023 Tc
0 0 Td
[()8()]TJ
/F8 1 Tf
5.5161 0 Td
(is)Tj
0.8107 0 Td
(the)Tj
1.4117 0 Td
(first)Tj
1.7121 0 Td
(gene)Tj
2.0012 0 Td
(of)Tj
0.9808 0 Td
(a)Tj
0.635 0 Td
(two-gene)Tj
3.7644 0 Td
(operon)Tj
2.982 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.7461 0 Td
(1A)Tj
0 g
(\),)Tj
1.8652 0 Td
(the)Tj
1.4173 0 Td
(second)Tj
2.897 0 Td
(of)Tj
0.9751 0 Td
(which,)Tj
/F14 1 Tf
2.761 0 Td
[()8()]TJ
/F8 1 Tf
(,)Tj
-31.4757 -1.2982 Td
(contains)Tj
3.4525 0 Td
(all)Tj
1.0942 0 Td
(of)Tj
0.9751 0 Td
(the)Tj
1.4116 0 Td
(canonical)Tj
3.8835 0 Td
(motifs)Tj
2.6475 0 Td
(of)Tj
0.9751 0 Td
(a)Tj
0.635 0 Td
(nucleotide-binding)Tj
7.5514 0 Td
(domain)Tj
3.2088 0 Td
(\(NBD\))Tj
2.8574 0 Td
(of)Tj
0.9807 0 Td
(an)Tj
1.1622 0 Td
(ABC)Tj
2.092 0 Td
(transporter)Tj
-32.927 -1.3039 Td
(\()Tj
0.83 0.64 0.02 0 k
(S1)Tj
1.4513 0 Td
(Fig)Tj
0 g
(\).)Tj
1.9502 0 Td
(Since)Tj
2.2677 0 Td
(both)Tj
1.9842 0 Td
(genes)Tj
2.3414 0 Td
(together)Tj
3.3846 0 Td
(are)Tj
1.3946 0 Td
(predicted)Tj
3.8268 0 Td
(to)Tj
0.9864 0 Td
(consist)Tj
2.8573 0 Td
(a)Tj
0.635 0 Td
(functional)Tj
4.1159 0 Td
(ABC)Tj
2.0919 0 Td
(transport)Tj
3.7531 0 Td
(system,)Tj
3.033 0 Td
(a)Tj
-36.0734 -1.2983 Td
(mutant)Tj
3.033 0 Td
(bearing)Tj
3.1011 0 Td
(deletions)Tj
3.651 0 Td
(of)Tj
0.9808 0 Td
(both)Tj
/F14 1 Tf
1.9842 0 Td
[()8()]TJ
/F8 1 Tf
5.5162 0 Td
(and)Tj
/F14 1 Tf
1.6724 0 Td
[()8()]TJ
/F8 1 Tf
5.5162 0 Td
(\()Tj
/F14 1 Tf
[()8()]TJ
/F8 1 Tf
6.7578 0 Td
(double)Tj
-32.2127 -1.2983 Td
(mutant\))Tj
3.3448 0 Td
(was)Tj
1.6328 0 Td
(prepared)Tj
3.6453 0 Td
(and)Tj
1.6667 0 Td
(tested)Tj
2.4265 0 Td
(for)Tj
1.3209 0 Td
(induction)Tj
3.9572 0 Td
(of)Tj
0.9694 0 Td
(type)Tj
1.8255 0 Td
(I)Tj
0.5329 0 Td
(interferons)Tj
4.4107 0 Td
(response)Tj
3.5716 0 Td
(in)Tj
0.9922 0 Td
(infected)Tj
3.2598 0 Td
(bone)Tj
2.1203 0 Td
(mar-)Tj
-35.6766 -1.3039 Td
(row)Tj
1.7234 0 Td
(derived)Tj
3.0841 0 Td
(macrophages)Tj
5.2951 0 Td
(\(BMDMs\).)Tj
4.4333 0 Td
(To)Tj
1.304 0 Td
(this)Tj
1.5987 0 Td
(end,)Tj
1.8652 0 Td
(total)Tj
1.9105 0 Td
(mRNA)Tj
3.0047 0 Td
(from)Tj
2.1317 0 Td
(macrophages)Tj
5.295 0 Td
(infected)Tj
3.2655 0 Td
(with)Tj
-34.9112 -1.2982 Td
(either)Tj
2.4151 0 Td
(the)Tj
/F14 1 Tf
1.4116 0 Td
[()8()]TJ
/F8 1 Tf
6.4403 0 Td
(mutant)Tj
3.033 0 Td
(or)Tj
1.0602 0 Td
(wild-type)Tj
3.8211 0 Td
(\(WT\))Tj
/F14 1 Tf
2.398 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F14 1 Tf
0.9355 0 Td
[()6( )11(\n)6( )4()-9()-15()11( )2(\r)2()2(\n)6()8()]TJ
/F8 1 Tf
5.8336 0 Td
(was)Tj
1.6441 0 Td
(extracted)Tj
3.7134 0 Td
(and)Tj
1.6724 0 Td
(IFN-)Tj
/F15 1 Tf
()Tj
/F8 1 Tf
-34.3783 -1.2983 Td
(and)Tj
1.6724 0 Td
(IL-6)Tj
1.8482 0 Td
(transcription)Tj
5.1873 0 Td
(levels)Tj
2.2621 0 Td
(were)Tj
2.0466 0 Td
(quantified)Tj
4.1158 0 Td
(6)Tj
0.6804 0 Td
(hours)Tj
2.4151 0 Td
(post-infection,)Tj
5.7656 0 Td
(using)Tj
2.296 0 Td
(real-time)Tj
3.6851 0 Td
(quantitative)Tj
-31.9746 -1.3039 Td
(PCR)Tj
2.0069 0 Td
(\(RT-qPCR\).)Tj
4.8699 0 Td
(Indeed,)Tj
3.101 0 Td
(the)Tj
/F14 1 Tf
1.4117 0 Td
[()8()]TJ
/F8 1 Tf
6.4403 0 Td
(mutant)Tj
3.033 0 Td
(elicited)Tj
2.948 0 Td
(a)Tj
0.635 0 Td
(much)Tj
2.4434 0 Td
(weaker)Tj
2.9367 0 Td
(type)Tj
1.8368 0 Td
(I)Tj
0.5443 0 Td
(interferon)Tj
-32.207 -1.2983 Td
(response)Tj
3.5659 0 Td
(in)Tj
0.9865 0 Td
(comparison)Tj
4.7791 0 Td
(to)Tj
0.9752 0 Td
(WT)Tj
1.7461 0 Td
(bacteria)Tj
3.2145 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.7347 0 Td
(1B)Tj
1.2246 0 Td
(and)Tj
1.6611 0 Td
(1C)Tj
0 g
(\),)Tj
1.8368 0 Td
(despite)Tj
2.897 0 Td
(identical)Tj
3.4753 0 Td
(replication)Tj
4.3029 0 Td
(rates)Tj
2.0013 0 Td
(between)Tj
-34.401 -1.2983 Td
(the)Tj
1.4116 0 Td
(two)Tj
1.6497 0 Td
(bacterial)Tj
3.4583 0 Td
(populations)Tj
4.7395 0 Td
(\()Tj
0.83 0.64 0.02 0 k
(Fig)Tj
1.7461 0 Td
(1D)Tj
0 g
(\).)Tj
1.9219 0 Td
(Collectively,)Tj
4.8529 0 Td
(these)Tj
2.16 0 Td
(results)Tj
2.6759 0 Td
(demonstrate)Tj
5.0229 0 Td
(that)Tj
/F14 1 Tf
1.7008 0 Td
[()8()]TJ
/F8 1 Tf
-31.3396 -1.2982 Td
(contributes)Tj
4.541 0 Td
(to)Tj
0.9865 0 Td
(the)Tj
1.4173 0 Td
(induction)Tj
3.9628 0 Td
(of)Tj
0.9751 0 Td
(the)Tj
1.4117 0 Td
(innate)Tj
2.6305 0 Td
(Type)Tj
2.1486 0 Td
(I)Tj
0.5443 0 Td
(interferon)Tj
4.0762 0 Td
(immune)Tj
3.5092 0 Td
(response)Tj
3.583 0 Td
(to)Tj
0.9865 0 Td
(intracellularly)Tj
-30.7727 -1.3039 Td
(growing)Tj
/F14 1 Tf
3.3958 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F14 1 Tf
0.9355 0 Td
[()6( )11(\n)6( )4()-9()-15()11( )2(\r)2()2(\n)6()8()]TJ
/F8 1 Tf
5.8336 0 Td
(bacteria.)Tj
ET
Q
q
1 j
1 J
0 w
11.9999 0 0 11.9999 200.0125 242.9858 cm
BT
/F7 1 Tf
1 TL
-0.0033 Tc
0 0 Td
(LMRG_022701)Tj
/F0 1 Tf
7.474 0 Td
(operon)Tj
3.293 0 Td
(encodes)Tj
3.9543 0 Td
(an)Tj
1.3134 0 Td
(ABC)Tj
2.263 0 Td
(importer)Tj
3.8457 0 Td
(of)Tj
1.0441 0 Td
(L-glutamine)Tj
ET
Q
q
1 j
1 J
0 w
10 0 0 10 200.0125 225.9779 cm
BT
/F8 1 Tf
1 TL
-0.004 Tc
0 0 Td
(In)Tj
1.1055 0 Td
(ABC)Tj
2.1486 0 Td
(transporters)Tj
5.0853 0 Td
(that)Tj
1.7745 0 Td
(function)Tj
3.6283 0 Td
(as)Tj
1.0148 0 Td
(importers,)Tj
4.3824 0 Td
(the)Tj
1.474 0 Td
(SBP)Tj
1.8311 0 Td
(is)Tj
0.8504 0 Td
(located)Tj
3.0784 0 Td
(extracellularly,)Tj
6.0605 0 Td
(where)Tj
2.6475 0 Td
(it)Tj
-35.0813 -1.2982 Td
(recognizes)Tj
4.422 0 Td
(the)Tj
1.4683 0 Td
(substrate)Tj
3.8041 0 Td
(with)Tj
1.9956 0 Td
(high)Tj
2.0069 0 Td
(affinity)Tj
3.067 0 Td
(and)Tj
1.7292 0 Td
(delivers)Tj
3.2995 0 Td
(it)Tj
0.7823 0 Td
(to)Tj
1.0262 0 Td
(the)Tj
1.4683 0 Td
(transmembrane)Tj
6.582 0 Td
(domain.)Tj
3.549 0 Td
(A)Tj
-35.2004 -1.2983 Td
(BLAST)Tj
3.1067 0 Td
(analysis)Tj
3.3279 0 Td
(of)Tj
1.0148 0 Td
(LMRG_02770)Tj
5.862 0 Td
(against)Tj
3.0273 0 Td
(the)Tj
/F14 1 Tf
1.4684 0 Td
[()]TJ
/F8 1 Tf
(.)Tj
/F14 1 Tf
0.9978 0 Td
[()7( )10()12()]TJ
/F8 1 Tf
1.559 0 Td
(K-12)Tj
2.194 0 Td
(genome,)Tj
3.634 0 Td
(revealed)Tj
3.5206 0 Td
(the)Tj
1.474 0 Td
(highest)Tj
3.0898 0 Td
(homol-)Tj
-34.2763 -1.3039 Td
(ogy)Tj
1.644 0 Td
(to)Tj
1.0262 0 Td
(several)Tj
2.9366 0 Td
(ABC)Tj
2.143 0 Td
(transporters)Tj
5.0853 0 Td
(\(importers\))Tj
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